Crystal structure of FIV dUTP pyrophosphatase
نویسندگان
چکیده
منابع مشابه
Structures of feline immunodeficiency virus dUTP pyrophosphatase and its nucleotide complexes in three crystal forms.
dUTP pyrophosphatase (dUTPase) cleaves the alpha-beta phosphodiester of dUTP to form pyrophosphate and dUMP, preventing incorporation of uracil into DNA and providing the substrate for thymine synthesis. Seven crystal structures of feline immunodeficiency virus (FIV) dUTPase in three crystal forms have been determined, including complexes with substrate (dUTP), product (dUMP) or inhibitor (dUDP...
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Genomes of all free-living organisms encode the enzyme dUTPase (dUTP pyrophosphatase), which plays a key role in preventing uracil incorporation into DNA. In the present paper, we describe the biochemical and structural characterization of DUT1 (Saccharomyces cerevisiae dUTPase). The hydrolysis of dUTP by DUT1 was strictly dependent on a bivalent metal cation with significant activity observed ...
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Single crystals of the yeast enzyme inorganic pyrophosphatase that are suitable for x-ray analysis have been grown. The space group of the crystals is P21. Their unit cell dimensions are: (I = 70.4, b = 95.4, c = 52.2 A, and fl = 99.8”. There is 1 dimeric enzyme molecule with a molecular weight of approximately 68,000 in the asymmetric unit of the unit cell. The crystal is approximately 57% pro...
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The reaction of [PPhJ, NOS,] with CuCl and KSCN (1 :3:3) in acetone produces the yell-ow crystals of [PPh,], [WOS,(CuNCS),]. The crystals are triclinic, space group P1(2), z=2, a=12.4823(7), b=12.9224(7), c=18.6395(10)?, ?=83.907(5), ?= 73.152(4), Y= 65.194(4)'. The crystal structure was determined by single crystal Xray diffraction methods (Mo-K ?) and refined by least-squares calculations ...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 1996
ISSN: 0108-7673
DOI: 10.1107/s0108767396094767